Gel Filtration Chromatography (GFC) – Definition, Principle, Types, Steps, Applications, Advantages & Limitations

Gel Filtration Chromatography (GFC) – Definition, Principle, Types, Steps, Applications, Advantages & Limitations

Gel Filtration Chromatography (GFC), also known as Size Exclusion Chromatography, is a separation technique used to isolate biomolecules based on size. Learn its principle, parts, steps, types, uses, advantages, and limitations in detail.

Gel Permeation Chromatography (GPC) – Definition, Principle, Instrumentation, Steps, Applications, Advantages & Limitations

Gel Permeation Chromatography (GPC) – Definition, Principle, Instrumentation, Steps, Applications, Advantages & Limitations

Gel Permeation Chromatography (GPC), also called Size Exclusion Chromatography (SEC) or Gel Filtration Chromatography, is a powerful separation technique widely used in biochemistry, biotechnology, and polymer science.

Flow Cytometry – Definition, Principle, Steps, Types, Applications, Advantages & Limitations

Flow Cytometry – Definition, Principle, Steps, Types, Applications, Advantages & Limitations

Flow cytometry is a powerful analytical technique widely used in biology, medicine, and research labs to study cells and particles in a fluid suspension.

Polyacrylamide Gel Electrophoresis (PAGE) – Principle, Procedure, Applications, Advantages & Limitations

Polyacrylamide Gel Electrophoresis (PAGE) – Principle, Procedure, Applications, Advantages & Limitations

Polyacrylamide Gel Electrophoresis (PAGE) is a laboratory technique used to separate proteins and nucleic acids based on their size and charge by making them move through a polyacrylamide gel under electricity.

Immunoelectrophoresis – Definition, Principle, Procedure, Results, Applications, Advantages & Limitations

Immunoelectrophoresis – Definition, Principle, Procedure, Results, Applications, Advantages & Limitations

Immunoelectrophoresis (IEP) is a powerful analytical technique that combines the principles of electrophoresis and immunodiffusion. In simple terms, it helps to separate and identify proteins (antigens) in a mixture by allowing them to migrate in an electric field and then testing them with specific antibodies.

Types of Spectroscopy – Principles, Types, Steps, and Applications

Types of Spectroscopy – Principles, Types, Steps, and Applications

Spectroscopy is the study of how matter interacts with electromagnetic radiation (light, X-rays, gamma rays, etc.). This interaction can involve absorption, emission, or scattering of radiation. By studying these processes, scientists can obtain valuable information about the composition, structure, and properties of substances.

Electron Spin Resonance (ESR) – Principle, Instrumentation, Working, Applications, Advantages & Limitations

Electron Spin Resonance (ESR) – Principle, Instrumentation, Working, Applications, Advantages & Limitations

Electron Spin Resonance (ESR), also known as Electron Paramagnetic Resonance (EPR) or Electron Magnetic Resonance (EMR), is a powerful spectroscopic technique used to study materials with unpaired electrons.

Pulsed Field Gel Electrophoresis (PFGE) – Definition, Principle, Steps, Applications, Advantages & Limitations

Pulsed Field Gel Electrophoresis (PFGE) – Definition, Principle, Steps, Applications, Advantages & Limitations

Pulsed Field Gel Electrophoresis (PFGE) is an advanced laboratory technique used to separate very large DNA molecules. Unlike standard gel electrophoresis, PFGE can separate DNA fragments greater than 50 kilobase pairs (kbp) up to several megabase pairs (Mb).

Chi-square Test in Research Methodology – Definition, Formula, Uses, and Examples

Chi-square Test in Research Methodology – Definition, Formula, Uses, and Examples

Learn the Chi-square test in research methodology with formula, types, conditions, examples, and FAQs. Easy guide for biology and health research.

Analytical Balance – Definition, Principle, Parts, Types, Working, Applications, Advantages & Precautions

Analytical Balance – Definition, Principle, Parts, Types, Working, Applications, Advantages & Precautions

An Analytical Balance is a highly sensitive laboratory instrument designed to measure the mass of substances with extreme accuracy. It is a Class II balance often referred to as a laboratory balance, capable of measuring solid, liquid, granular substances, or powders.