Gel Permeation Chromatography (GPC) – Definition, Principle, Instrumentation, Steps, Applications, Advantages & Limitations

Gel Permeation Chromatography (GPC) – Definition, Principle, Instrumentation, Steps, Applications, Advantages & Limitations

Gel Permeation Chromatography (GPC), also called Size Exclusion Chromatography (SEC) or Gel Filtration Chromatography, is a powerful separation technique widely used in biochemistry, biotechnology, and polymer science.

Thin Layer Chromatography (TLC) – Principle, Steps, Applications, Advantages & Limitations

Thin Layer Chromatography (TLC) – Principle, Steps, Applications, Advantages & Limitations

Thin Layer Chromatography (TLC) is a simple, quick, and effective technique used to separate and identify the components of a mixture. It is performed on a plate coated with a thin layer of adsorbent material (e.g., silica gel, alumina, or cellulose) which acts as the stationary phase.

Gas Chromatography (GC) – Principle, Parts, Procedure, Steps, Applications, Advantages & Limitations

Gas Chromatography (GC) – Principle, Parts, Procedure, Steps, Applications, Advantages & Limitations

Gas Chromatography (GC) is a powerful analytical technique used to separate, identify, and quantify compounds that can exist in the gaseous phase.

Ion Exchange Chromatography – Principle, Instrumentation, Procedure, Applications

Ion Exchange Chromatography – Principle, Instrumentation, Procedure, Applications

Ion Exchange Chromatography is a powerful separation technique based on charge differences of molecules. Learn the principle, types of exchangers, instrumentation, working steps, applications, advantages, and limitations of ion exchange chromatography.

Adsorption Chromatography – Principle, Types, Procedure, Applications & Advantages

Adsorption chromatography column showing stationary and mobile phases

Adsorption chromatography is a type of liquid-solid or gas-solid chromatography where compounds are separated based on how strongly they bind (adsorb) to a solid surface.

Affinity Chromatography – Principle, Components, Procedure, Applications & Advantages

Diagram of affinity chromatography showing ligand-target binding, Affinity chromatography column setup with matrix, ligand, and mobile phase

Learn about affinity chromatography, its principle, components, procedure, types, applications, advantages, and limitations in biology and biotechnology.

Chromatography – Principle, Types, Steps, Uses, and Advantages

Chromatography: Principle, Types, Steps, Applications & Advantages

Learn about chromatography, its principle, types, steps, and applications in biology, research, pharmaceuticals, food safety, and forensic science.