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Limitations of Pulsed Field Gel Electrophoresis

Pulsed Field Gel Electrophoresis (PFGE) – Definition, Principle, Steps, Applications, Advantages & Limitations

October 23, 2025September 8, 2025 by mayankmahara15@gmail.com
Pulsed Field Gel Electrophoresis (PFGE) – Definition, Principle, Steps, Applications, Advantages & Limitations

Pulsed Field Gel Electrophoresis (PFGE) is an advanced laboratory technique used to separate very large DNA molecules. Unlike standard gel electrophoresis, PFGE can separate DNA fragments greater than 50 kilobase pairs (kbp) up to several megabase pairs (Mb).

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