Thin Layer Chromatography (TLC) – Principle, Steps, Applications, Advantages & Limitations

Thin Layer Chromatography (TLC) – Principle, Steps, Applications, Advantages & Limitations

Thin Layer Chromatography (TLC) is a simple, quick, and effective technique used to separate and identify the components of a mixture. It is performed on a plate coated with a thin layer of adsorbent material (e.g., silica gel, alumina, or cellulose) which acts as the stationary phase.

UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages & Limitations

UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages & Limitations

UV-Vis Spectroscopy (Ultraviolet–Visible Spectroscopy) is a widely used analytical technique that studies how a sample absorbs or transmits ultraviolet (190–400 nm) and visible light (400–800 nm). It is also called absorption spectroscopy or reflectance spectroscopy.

Fluorimetry – Principle, Instrumentation, Factors, Applications, Advantages & Limitations

Fluorimetry – Principle, Instrumentation, Factors, Applications, Advantages & Limitations

Fluorimetry is an analytical technique used to measure the fluorescent light emitted by a substance when it absorbs ultraviolet (UV) or visible light. It helps in studying the concentration, properties, and interactions of compounds in a sample.

Spectrophotometer – Principle, Components, Applications, Advantages & Limitations

Spectrophotometer – Principle, Instrumentation, Applications, Advantages & Limitations

A spectrophotometer is a scientific instrument used to measure how much light a sample absorbs. It is mainly used to determine the concentration of solutes in a solution.

Gas Chromatography (GC) – Principle, Parts, Procedure, Steps, Applications, Advantages & Limitations

Gas Chromatography (GC) – Principle, Parts, Procedure, Steps, Applications, Advantages & Limitations

Gas Chromatography (GC) is a powerful analytical technique used to separate, identify, and quantify compounds that can exist in the gaseous phase.

pH Meter – Principle, Parts, Working, Procedure, Types, Applications, Advantages & Precautions

pH Meter – Principle, Parts, Working, Procedure, Types, Applications, Advantages & Precautions

A pH meter is a modern electronic device that measures hydrogen ion concentration in a solution and displays it directly as a pH value.

Laminar Flow Hood / Cabinet – Principle, Types, Parts, Procedure, Applications & Precautions

Laminar Flow Hood / Cabinet – Principle, Types, Parts, Procedure, Applications & Precautions

A Laminar Flow Hood (also called Laminar Flow Cabinet) is a special enclosed workstation designed to maintain a contamination-free environment in laboratories.

Agarose Gel Electrophoresis – Principle, Procedure, Applications, Advantages & Limitations

Agarose Gel Electrophoresis – Principle, Procedure, Applications, Advantages & Limitations

Agarose Gel Electrophoresis is one of the most widely used techniques in molecular biology, genetics, and biochemistry. It plays a central role in DNA and RNA analysis, genetic fingerprinting, and even in medical diagnostics. This method allows scientists to separate and visualize nucleic acids (DNA and RNA) based on their size and charge.

Hot Air Oven – Principle, Parts, Types, Working, Applications & Advantages

Hot Air Oven – Principle, Parts, Types, Working, Applications, Advantages

Hot Air Oven is a laboratory sterilization equipment based on dry heat. Learn its principle, parts, types, working steps, applications in microbiology, advantages, limitations, and safety precautions.

Instruments Used in Microbiology Laboratory – Principles, Uses, and Applications

Instruments Used in Microbiology Laboratory – Principles, Uses, and Applications

Learn about the major instruments used in microbiology labs, including their principles, working, and applications. Covers autoclave, microscope, centrifuge, spectrophotometer, incubator, pH meter, laminar hood, and more.