UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages & Limitations

UV-Vis Spectroscopy – Principle, Instrumentation, Applications, Advantages & Limitations

UV-Vis Spectroscopy (Ultraviolet–Visible Spectroscopy) is a widely used analytical technique that studies how a sample absorbs or transmits ultraviolet (190–400 nm) and visible light (400–800 nm). It is also called absorption spectroscopy or reflectance spectroscopy.

Fluorimetry – Principle, Instrumentation, Factors, Applications, Advantages & Limitations

Fluorimetry – Principle, Instrumentation, Factors, Applications, Advantages & Limitations

Fluorimetry is an analytical technique used to measure the fluorescent light emitted by a substance when it absorbs ultraviolet (UV) or visible light. It helps in studying the concentration, properties, and interactions of compounds in a sample.